Journal: bioRxiv

Article Title: Human Assembloid Model of Emergent Neurotropic Enteroviruses

doi: 10.1101/2025.11.18.689148

Figure Lengend Snippet: (A) Representative PHOX2B immunostainings of hSpO infected with PV, EV-D68, or EV-A71 at indicated days post infection. Scale bar, 50 μm. (B) Number of PHOX2B positive cells per hSpO area for PV, EV-D68, or EV-A71 infected hSpO. n= 19 PV infected hSpO, n= 20 EV-D68 infected hSpO, n= 18 EV-A71 infected hSpO from 2 hiPS cell lines from 2 differentiations. Values represent mean ± SEM. P-values were determined by two-way ANOVA (adjusted with Benjamini–Hochberg). *P < 0.05, **P < 0.01, ****P < 0.0001. (C) Damage bins as related to , with individual values graphed. n= 9 PV infected hSpO from 2 hiPS cell lines from 1 differentiation, n= 7 EV-D68 infected hSpO from 2 hiPS cell lines from 2 differentiations, n= 5 EV-A71 infected hSpO from 2 hiPS cell lines from 2 differentiations. Datasets represent mean ± SEM. P-values were determined by two-way ANOVA (adjusted with Benjamini–Hochberg). **P < 0.01, ****P < 0.0001.

Article Snippet: The primary antibodies used to detect the presence of the indicated proteins in this study are as described: dsRNA clone rJ2 (MABE1134; 1:50; Millipore), EV-D68 VP1 (GTX132313; 1:200; Genetex), enterovirus pan monoclonal antibody L66J (MA5-18206; 1:50; Thermo Fisher Scientific), Desmin (50-173-1004; 1:200; Thermo Fisher Scientific), cleaved caspase-3 (9661; 1:400; Cell Signaling Technology), GFAP (13-0300; 1,000; Thermo Fisher Scientific), PHOX2B (AF4940-SP; 1:2,000; R&D Systems), MAP2 (188 004; 1:5,000; Synaptic Systems) and SPARCL1 (AF2728; 1:300; R&D Systems).

Techniques: Infection